Cell Biology
Hemocytometer Cell Count & Viability
Concentration (cells/mL) = (Count × Dilution × 10⁴) / Squares; Viability (%) = (Live / Total) × 100
Target Output: Cell Concentration (C)[SOLVED]
1000000
Calculated concentration of cells
cells
Total number of cells counted
cells
Number of viable cells counted
cells
Number of non-viable cells counted
sq
Number of large hemocytometer squares counted
x
Dilution factor (e.g., 2 for 1:1 with trypan blue)
💡 What This Is Useful For: Determining cell concentration for seeding cell culture experiments, passaging cells, and preparing single-cell suspensions.
📐 Real-World Worked Example: Counting 180 live cells and 20 dead cells in 4 squares with a 2x dilution factor: Concentration = (200 * 2 * 10^4) / 4 = 1,000,000 cells/mL.
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Scientifically reviewed by:D Yap, MBiochem (Oxon), DIC, PhD (Lond)
Clinical Medicine Research Scientist·LinkedIn Profile ↗·ORCID: 0000-0002-5370-4592 ↗
⚠️ Research Reference Notice: For reference and research education only. Reviewer is a clinical medicine research scientist and not a practicing physician or veterinarian, and does not provide clinical certification or medical advice. Experimental drug doses and clinical measurements must be verified against institutional protocols, primary manufacturer labeling, and physician orders.