Lab Chemistry
Solution Dilution (C1V1 = C2V2)
Concentration₁ × Volume₁ = Concentration₂ × Volume₂
Target Output: Initial Concentration (Stock) (C₁)[SOLVED]
50
Concentration of concentrated stock solution
Volume of stock to pipette into final tube
Desired concentration in experiment
Total volume after adding diluent/buffer
💡 What This Is Useful For: Daily protocol across biomedical labs: preparing 1× PBS from 10× stock, preparing micromolar compound dilutions from 10 mM DMSO stocks, and diluting primary antibodies for Western blots.
📐 Real-World Worked Example: To prepare 50 mL (V₂) of 2 mM (C₂) drug solution from a 50 mM (C₁) stock: V₁ = (2 × 50) / 50 = 2 mL stock, diluted with 48 mL vehicle/buffer.
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Scientifically reviewed by:D Yap, MBiochem (Oxon), DIC, PhD (Lond)
Clinical Medicine Research Scientist · LinkedIn Profile ↗
⚠️ Research Reference Notice: For reference and research education only. Reviewer is a clinical medicine research scientist and not a practicing physician or veterinarian, and does not provide clinical certification or medical advice. Experimental drug doses and clinical measurements must be verified against institutional protocols, primary manufacturer labeling, and physician orders.