Drug Formulation

Compound, RMM & Dose Basis

Shared by the stock solution and in vivo formulation calculators below. Import an approved compound from the RMM / RFM calculator or enter it from the vial / CoA.

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Scientifically reviewed by:D Yap, MBiochem (Oxon), DIC, PhD (Lond)

Clinical Medicine Research Scientist·LinkedIn Profile ↗·ORCID: 0000-0002-5370-4592 ↗

⚠️ Research Reference Notice: For reference and research education only. Reviewer is a clinical medicine research scientist and not a practicing physician or veterinarian, and does not provide clinical certification or medical advice. Experimental drug doses and clinical measurements must be verified against institutional protocols, primary manufacturer labeling, and physician orders.

Import from RMM / RFM calculator (no approved compound shared yet)Open RMM / RFM calculator

Compound

RMM as supplied (g/mol)

RMM active moiety (g/mol)

Purity (%)

Mass doses and mg/mL concentrations refer to:The material as supplied/weighed — no salt correctionThe active moiety (free base / acid) — mass corrected by RMM_supplied ÷ RMM_active = 1×

I have confirmed the RMM, salt form, purity and dose basis against the vial label or Certificate of Analysis.

Drug Formulation

Stock Solution Preparation

Solvent volume to dissolve a whole vial, or mass to weigh for a target concentration and volume. Molar stocks use RMM as supplied; mg/mL stocks follow the dose basis.

Mass in vial (mg)

Target concentration

🔒 Complete and confirm the compound panel above to show results.

Drug Formulation

Serial Dilution & Dose-Response Planner

Equal-fold series with constant vehicle: each tube keeps V after passing T = V ÷ (fold − 1) to the next.

Unit (label)

Stock (µM)

Top concentration (µM)

Fold per step

Points

Volume kept per tube (µL)

Solvent in stock (% v/v)

100 for a neat DMSO stock

Added to assay at 1 : (×)

e.g. 10 µL into 90 µL = 10

Max solvent in assay (%)

Tube 1: stock0.15 µL

Tube 1: diluent149.8 µL

Tubes 2…n: diluent each100 µL

Transfer between tubes50 µL

Solvent in diluent (keeps vehicle constant)0.1%

Solvent in assay0.01%

Discard 50 µL from the last tube so every tube holds 100 µL.

⚠️ Tube 1 needs only 0.15 µL of stock, below reliable pipetting. Make an intermediate dilution of the stock first.

Tube	In tube (µM)	In assay (µM)
1	10	1
2	3.333	0.3333
3	1.111	0.1111
4	0.3704	0.03704
5	0.1235	0.01235
6	0.04115	0.004115
7	0.01372	0.001372
8	0.004572	0.0004572

Preclinical Formulation

In Vivo Cohort Formulation

Fixed dose-volume dosing: working concentration = dose ÷ dose volume. Total volume, mass to weigh and vehicle component volumes for a whole cohort.

1. Study design

Species

Route

Dose (mg/kg)

Dose volume (mL/kg)

Mouse oral gavage good practice ≤ 10 mL/kg

Animals

Mean body weight (g)

Doses per animal from this batch

Overage (%)

Syringe dead volume and losses

2. Vehicle

Preset

1%

2%

3%

4%

Solubility in vehicle (mg/mL, optional)

🔒 Complete and confirm the compound panel above to show results.