UV Irradiance, Fluence & Photodosimetry Suite

Calibrated optical radiation calculations, crosslinker photodiode aging drift, and quantum photon flux density.

Target Variable to Solve:

Measured Irradiance (Eₑ)

From calibrated radiometer sensor

Exposure Duration (t)

Total elapsed shutter/lamp run-time

Target: Delivered Fluence

Total radiant exposure delivered

Delivered Radiant Exposure (Fluence)

26.46 mJ/cm2

Standard Lab Dose:26.46 mJ/cm²

High-Dose / Solar:0.02646 J/cm²

SI Base Fluence:264.6 J/m²

Equivalent Run-Time:14 s

Peer-Reviewed Cancer Research

The UVA-Induced Single-Base Mutational Signature of CX-5461 in Human Cells

Zaikova, Yap, Sarvar, Hafezi, Tan, Cerda, Li, Lai, Gelmon, Hilton, Seymour, Stirling, Cescon, Aparicio. Nucleic Acids Research, 2026. Demonstrating that quinolone-derived anti-cancer agent CX-5461 undergoes UVA-mediated photoactivation to generate a unique somatic T>A and T>C mutational profile.

View Paper↗

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Scientifically reviewed by:D Yap, MBiochem (Oxon), DIC, PhD (Lond)

Clinical Medicine Research Scientist (Aparicio Lab Co-Author)·LinkedIn Profile ↗·ORCID: 0000-0002-5370-4592 ↗

Reviewed for: UV photobiology and crosslinker irradiance calculations

⚠️ Research Reference Notice: For reference and research education only. Reviewer is a clinical medicine research scientist and not a practicing physician or veterinarian, and does not provide clinical certification or medical advice. For laboratory photobiology, crosslinker calibration, and preclinical genomics research.

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Photobiology & Cancer Photogenomics Metrology

Why UV Crosslinkers Drift and Why Calibrated Radiometry is Essential

Derived from the methodology of Zaikova, Yap, Aparicio et al., Nucleic Acids Research (2026).

1. The Fundamental Dose Law: Fluence (H) = Irradiance (Eₑ) × Time (t)

In optical radiation metrology (CIE S 026/E:2018, ISO 21348), the photochemical effect of ultraviolet light on cellular chromophores is determined by radiant exposure (also termed fluence or dose, denoted H or D). It is defined as the time integral of irradiance:

H [mJ/cm²] = Eₑ [mW/cm²] × t [seconds]

To deliver 26.5 mJ/cm² of UVA (365 nm) when your lamp produces an incident radiant flux density of 1.930 mW/cm², the required exposure duration is:

t = 26.46 mJ/cm² ÷ 1.930 mW/cm² = 13.71 seconds